منابع مشابه
Cohomology of Cryo-Electron Microscopy
The goal of cryo-electron microscopy (EM) is to reconstruct the 3-dimensional structure of a molecule from a collection of its 2-dimensional projected images. In this paper, we show that the basic premise of cryo-EM—patching together 2-dimensional projections to reconstruct a 3-dimensional object—is naturally one of Čech cohomology with SO(2)-coefficients. We deduce that every cryoEM reconstruc...
متن کاملMathematics for cryo - electron microscopy
Single-particle cryo-electron microscopy (cryo-EM) has recently joined X-ray crystallography and NMR spectroscopy as a high-resolution structural method for biological macromolecules. Cryo-EM was selected by Nature Methods as Method of the Year 2015, large scale investments in cryo-EM facilities are being made all over the world, and the Nobel Prize in Chemistry 2017 was awarded to Jacques Dubo...
متن کاملCryo-Transmission Electron Microscopy Confirms Controlled Synthesis of Cadmium Sulfide Nanocrystals within Lecithin Vesicles
Size-quantized CdS nanocrystals of controlled dimensions are synthesized within monodisperse egg lecithin vesicles made by detergent depletion. Cryo-transmission electron microscopy (cryo-TEM) enables imaging in situ both of the vesicle wall and of the encapsulated nanocrystal. Cryo-TEM images confirm that only one particle per vesicle forms and that nanocrystal diameter is determined by the nu...
متن کامل(Cryo)Transmission Electron Microscopy of Phospholipid Model Membranes Interacting with Amphiphilic and Polyphilic Molecules
Lipid membranes can incorporate amphiphilic or polyphilic molecules leading to specific functionalities and to adaptable properties of the lipid bilayer host. The insertion of guest molecules into membranes frequently induces changes in the shape of the lipid matrix that can be visualized by transmission electron microscopy (TEM) techniques. Here, we review the use of stained and vitrified spec...
متن کاملCryo-transmission electron microscopy structure of a gigadalton peptide fiber of de novo design.
Nature presents various protein fibers that bridge the nanometer to micrometer regimes. These structures provide inspiration for the de novo design of biomimetic assemblies, both to address difficulties in studying and understanding natural systems, and to provide routes to new biomaterials with potential applications in nanotechnology and medicine. We have designed a self-assembling fiber syst...
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ژورنال
عنوان ژورنال: Nature
سال: 1982
ISSN: 0028-0836,1476-4687
DOI: 10.1038/299386c0